MonoclonalAntibodytoHumanTopoisomeraseIIIAlphaDescription
ThismonoclonalantibodywasraisedagainstpurifiedrecombinantproteinandisspecificfortheC-terminalregion(residues748-976). Theantibodyisnotidealforuseinwesternblottingexperimentsbutwillworkonslotblotapplications. Itiswellsuitedforimmunohistochemistrywithparaffinembeddedtissueusing1:25to150dilutions,thehightemperatureunmaskingprocedureandstandardABCtechniques. Itwillworkwithfrozentissuealthoughacetonefixationisrecommended. Wegenerallyrecommend60minincubationat25Cfortheprimaryantibody. SpecificconditionsusedineachlabmayvaryaswillspecificapplicationsforthisAB. Forthisreason,theconditionsfordetectingnuclearandnucleolarlocalizationshouldbeworkedoutempericallyineachlaboratory.

IgGClass: IgG3

StainingPattern: Nuclearandnucleolar

TopoIIIaBackgoundInformation:
DNAtopoIIIisatypeIAtopoisomerasethatissimilartotheprokaryotic(E.coli)topoI(omegaprotein). Thereactionmechanismsforthesetwoaresimilar. TheprototypictopoIinanimalcellsisatypeIBenzyme. TopoIIIahasbeenlocalizedtochromosome17(17p11.2-12)andencodesa976residue,110kDapolypeptide. Thebiochemicalfeaturesofthisenzymearenotwellunderstoodalthoughitislikelythatitbehavessimilarlytotheprokaryotichomologue(omegaprotein)initsstrongpreferencefornegativelysupercoiledDNAsubstrates.

Vialcontents:
Purifiedantibody(50ul)issuppliedin15mMsodiumazideinsterilewater.


MaterialSafetyData:
THISPRODUCEISNOTLICENSEDORAPPROVEDFORADMINISTRATIONTOHUMANSORTOANIMALSOTHERTHANEXPERIMENTALANIMALS. ForResearchOnly.

References:
Goulaouicetal. PurificationandcharacterizationofhumanDNAtopoisomeraseIII. NucleicAcidsResearch27:2443-2450(1999)
LiandWang. HumanDNAtopoIIIaisessentialinearlyembyrogenesis. PNAS95:1010-1013(1998)
Hanaietal. HumanTOP3: AsinglecopygeneencodingDNAtopoisomeraseII.PNAS93:3653-3657(1996)

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